Chlorophyll extraction from green leaves
Lab 1
November 4, 2016
Josy Chapman Jadarius McKinley 1000000
November 4, 2016
Josy Chapman Jadarius McKinley

Dark green leaves of cabahe plant
Paper towel
Mortar and pestle
Grind machine
2 plastic test tubes with lids and one 1.5ml test tube
95% ethanol
Put dry leaf pieces in the mortar and grind them. Continue until they turn into uniform yellow-green powder.
Label tubes with marker 1, and 2. Put powder in a test tube with tight lid. Add 5ml water in tube 1, and add 5ml 75% ethanol in tube2, close the lid and shake to extract chlorophyll.
In tube 2, there should be emerald-green slightly opalescent liquid on top of dark green powder (which will sink to the bottom of the test tube). This is it. Chlorophyll is extracted. Now, it's diluted solution contaminated with fine debris from the broken cells. Transfer 1.5 top liquid into test tubes, and take a picture.
What types of solution can dissolve chlorophyll? Why? Chlorophyll is readily soluble in alcohol, mostly insoluble in non polar alkanes like butane and hexane, and has some special relationships with polar water, because of its polar and ionic groups.
When we extracted chlorophyll, why can't we use water? Non polar solvents for obvious reasons won't either and also won't form much of an emulsion with alcohols in their bent state because; the alcohol is denser and forms a micelles like layer to protect itself from the charged Na+. If there is an excess of alcohol it will start forming an emulsion layer at the upper interface.
How many types of chlorophylls are in plant leaves? The chlorophylls, a and b, are the pigments of photosynthesis.
Why do we need to put fresh leaves in boiling water before to extract chlorophyll?Boiling water kills the cells and destroys enzymes which can promote chlorophyll degradation. It also breaks chloroplasts which makes chlorophyll extraction easier.

Conclusion: In tube 1, the top liquid color will be much lighter than tube 2. Because chlorophyll don't dissolve in water.