Lab 3 Respiration
Cellular respiration is a process of metabolic reactions in an organism\'s cells that converts biochemical energy from nutrients into adenosine triphosphate otherwise known as ATP as well as the release of waste products. Aerobic respiration is a type of cellular respiration that generates energy by oxidation of nutrients through krebs cycle where oxygen is the final electron acceptor. This process occurs in most cells everywhere, it\'s stages are Glycolysis, Krebs cycle, Electron Transport Chain and its products are CO 2 , H 2 0, and ATP. In this experiment we tested the effects of temperature on the aerobic respiration germinating peas. The hypothesis for this experiment is that respiration rate is positively correlated with the effects of temperature. One prediction is that oxygen consumption will be higher through units of time at room temperature water than that in ice water.
The experiment started by filling two beakers up with water, One with room temperature water and another with ice water and covered them both with a wooden support that would hold the two test tubes. The test tubes were then taken and each filled with ten peas each. They are then filled with a thin layer of cotton over the peas. The test tubes were then placed through the wooden support and into the beakers (one in each beaker) and left to adjust to the temperature of the water for eight minutes. After eight minutes a small layer of potassium hydroxide (KOH pellets) are placed into each test tube over the cotton layer. A rubber stopper with the attached capillary tubing with syringes and a pipette is then quickly applied to the openings of each test tube. The syringe attached to the tubing is then used to pull up a small amount of food dye into the pipette of each test tube and the dye stopped perfectly at the 0.8ml mark of each pipette. Once this is done we began recording the position of the dye in the pipette every thirty seconds for ten minutes. This came to 20 measurements in total which included the initial starting position.
The oxygen consumption for our personal experiment yielded unexpected results. The aerobic respiration in the germinating ice water peas initially began drastically consuming more oxygen per unit of time faster than the aerobic respiration in the room temperature peas.

Though the initial results of the experiment were unexpected the results toward the end of the experiment show the trend of the expected outcome. The germinating ice water peas began to drastically slow down its oxygen consumption and especially over the last nine recordings of measurements. The trend in the room temperature water was generally consistent throughout catching up to to the dye in the ice water pipette on the final measurement. This unusual outcome could have been due to an error in our preparation of the materials for the experiment. One error that could have affected the experiment I noticed is that after we had a problem with our capillary tubing on our first attempt at the experiment we then started over and proceeded with all procedures properly aside from being positive the full eight minutes of adjustment the test tubes were making to the water temperature was made. This is believe most likely explains this unusual trend in our results initially.
The class results generated varying results. This could also be due to user errors during the experiment or during calculation and interpretations of data.

According to our personal results and the class results I believe it is clear temperature and respiration rate of oxygen is positively correlated with each other. Though it is clear this experiment had much more varied and unusual results than usual I believe that much of it can be correlated to a user error in the experiment such as our initial results in the beginning of our experiment with the trend of the ice water peas consuming oxygen faster than the room temperature. Another possibility could have been an error in inputting the data. The trend when under optimal conditions showed oxygen consumption in room temperature water of the aerobic respiration of germinating peas to be faster and consistent. The trend for the ice water of the germinating peas while consistent if