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DATE

TITLE OF LAB

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Purpose
This experiment was conducted in order to compare the respiration rate in germinating soya seeds and that of non-germinating soya beans. The hypothesis under test was: germinating seeds have a higher cellular respiration than non-germinating seeds.
Introduction
More energy is generated by cellular respiration than yeast fermentation. In presence of oxygen, the pyruvate which is a product of glycolysis of glucose is oxidized in the mitochondrion to give 36 ATP molecules per glucose molecule. Other by prod ucts include carbon dioxide and water.
Materials
Glass beads
Cotton wool
Polyester fluffs
2 Volumeters
25 non-germinating soya beans
25 germinating soya beans
15% potassium hydroxide solution


Methods
In order to meet the purpose of the experiment and to prove our hypothesis, b oth the germinating and non-germinating soya beans were placed differently in three different Volumeters. A cotton ball that has been soaked in the solution of potassium hydroxide in order to eradicate the carbon dioxide available present in the air. The seeds are placed between absorbent cotton to prevent them from the solution. Glass beads are added on the Volumeter wit h non-germinating soybeans till the levels of the soy seeds + beads in the above two Volumeters are the same. A control experiment is now set. A third Volumeter full of glass beads is now set and the volume change in the 3 Volumeters is observed nearly for 20 min ute s and recorded as shown .









Results
For a period of 20 minutes, the observations of the volume change were recorded as follows:
Time(min)
germinating beans
Non-germinating beans
glass beads
5
8ml
8ml
6ml
10
7ml
8ml
6.4ml
15
6.5ml
8ml
6.3ml
20
5.9ml
8ml
6.8ml
25
3ml
8ml
6.9ml
Volume change
(mil/min)

65
86
90




Discussion
The rate of change in the three volumeters was seen to be different and was observed to be highest in the Volumeter with the germinating beans. This is because germination utilizes a lot of energy during cell division and differentiation. The Volumeter with non-germinating soya beans had little or no volume change . This was because no metabolic reactions are taking plac e hence little or no energy was needed. That was the same case to the Volumeter with glass beads since it has no living cells. Any observable change in this volumeter was d ue to the changes to pressure changes but this is the same effect in the other two Volumeters. The volume change rate is used to determine the rate of cellular respiration.
Conclusion
Basing on the experimental data, the germinating soya beans require more oxygen as compared to the non-germinating beans. This is because the germinating beans are undergoing through aerobic respiration and therefore require more energy to meet t he demands of the cellular respiration. Non-germinating soybeans have little or no cellular respiration and therefore they require little energy and hence require little oxygen .









References
Flare, B. (2003).  Cite a Website - Cite This For Me .  Uts c.utoronto.ca . Retrieved 14 July 2017, from http://www.utsc.utoronto.ca/pnda/SR/BGYA01/Respiration
Is there a difference in respiration rates of Germinating and Non-Germinating Peas . (2001).  Spot.colorado.edu . Retrieved 14 July 2017, from http://spot.colo rado.edu/basey/p.htm